The PathHunter® Dimerization Bioassay detects ligand induced dimerization of two subunits of a receptor-dimer pair.The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA).Cytoplasmic tail may have been deleted from one or both receptors.Binding of an agonist to one receptor subunit induces it to interact with its dimer partner,forcing complementation of the two enzyme fragments.This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.
Target/Description
Epidermal growth factor receptor
Group
Receptor Tyrosine Kinase
Cell Background
U2OS
Species
Human
Accession Number
NM_005228.3
Agonist/Ligand
Recombinant Human EGF
Antagonist/Inhibitor
Vectibix (Panitumumab)
Assay Measures
EGFR/ErbB2 Dimerization
Bioassay Target Name
EGFR/ErbB2
Assay Qualified With
Vectibix®
Drug Trademark Owner
Vectibix® is a registered trademark of Amgen Inc.