The PathHunter® Dimerization Bioassay detects ligand induced dimerization of two subunits of a receptor-dimer pair.The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA).Cytoplasmic tail may have been deleted from one or both receptors.Binding of an agonist to one receptor subunit induces it to interact with its dimer partner,forcing complementation of the two enzyme fragments.This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.
Target/Description
Interleukin 1 receptor, type I
Group
Interleukin Receptor
Cell Background
U2OS
Species
Human
Accession Number
NM_000877.3
Agonist/Ligand
IL-1β
Assay Measures
Dimerization
Bioassay Target Name
IL1R1-IL1RAP
Assay Qualified With
Kineret®
Drug Trademark Owner
Kineret®is a registered trademark of Swedish Orphan Biovitrum AB