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主营:G蛋白偶联受体,激酶,蛋白酶,核激素受体,细胞因子,转录因子和分泌蛋白。
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DiscoverX/PathHunter® Darbepoetin Alfa Bioassay Kit/200dp(2x96-well)/93-0965Y3-00019

DiscoverX/PathHunter® Darbepoetin Alfa Bioassay Kit/200dp(2x96-well)/93-0965Y3-00019
  • DiscoverX/PathHunter® Darbepoetin Alfa Bioassay Kit/200dp(2x96-well)/93-0965Y3-00019
商品介绍
The PathHunter® Dimerization Bioassay detects ligand induced dimerization of two subunits of a receptor-dimer pair.The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA).Cytoplasmic tail may have been deleted from one or both receptors.Binding of an agonist to one receptor subunit induces it to interact with its dimer partner,forcing complementation of the two enzyme fragments.This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.
Target/Description
Erythropoietin receptor
Group
Cytokine Receptor
Cell Background
U2OS
Species
Human
Accession Number
NM_000121.3
Agonist/Ligand
Erythropoietin
Assay Measures
Dimerization
Bioassay Target Name
EpoR-EpoR
Assay Qualified With
Aranesp®
Drug Trademark Owner
Aranesp® is a registered trademark of Amgen Inc.
How it works
品牌介绍

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DiscoverX/PathHunter® Aflibercept Bioassay Kit/93-0996Y1-00006/1000dp(10x96-well)93-0996Y1-00006细胞代谢8251.00
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