PathHunter® Bioassay kits provide a robust, highly reproducible and easy-to-use, cell-based functional assay to study receptor activity through a chemiluminescent output.The bioassay kits contain the reagents needed for a functional assay including ready-to-assay cells, plating, dilution & detection reagents, positive control agonist and assay plates. The ready-to-assay kits are designed to accelerate and simplify development of a QC potency assay or a NAb assay for biologic drugs.
Target/Description
Growth hormone receptor
Group
Cytosolic Tyrosine Kinase
SH2 Domain
PLCG1
Cell Background
U2OS
Species
Human
Accession Number
NM_000163
Agonist/Ligand
Recombinant Human Growth Hormone
Assay Measures
SH2 Recruitment
Bioassay Target Name
GHR-JAK1
Assay Qualified With
hGH
How it works
The above graph is representative dose-response curve with hGH. Using the protocol outlined in the user manual, the data show a potent, dose-dependent phosphorylation of the receptor by the cytosolic tyrosine kinase JAK1 in U2OS cells when treated with a dose-response of hGH.